Lactate Assay:Article Title: Type I interferon governs immunometabolic checkpoints that coordinate inflammation during Staphylococcal infection.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lysostaphin Sigma Cat#L7386 Critical commercial assays Cut & Run kit Cell Signaling Cat#86652 Lactate assay Cayman Chemical Cat#600450 Greiss reagent Abcam Cat#ab234044 Mito Stress Test Agilent Cat#103015-100 LookOut Mycoplasma PCR Detection Kit Sigma Cat#MP0035 MitoTracker Green Thermo Fisher Scientific Cat#M7514 MitoTracker Deep Red Thermo Fisher Scientific Cat#M22426 Propidium iodide Bio-Rad Cat#1351101 Hoechst Thermo Fisher Scientific Cat#H3570 DAPI Thermo Fisher Scientific Cat#EN62248 PowerUP SYBR Green PCR master mix Thermo Fisher Scientific Cat#A25742 RNAeasy Plus Mini kit Qiagen Cat#74134 iScript cDNA synthesis kit Bio-Rad Cat#1708890 BioMasher II homogenizers Thermo Fisher Scientific Cat#50-136-7690 Halt protease and phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RevertTM 700 Total Protein Stain for Western Blot Normalization LI-COR Cat#926-11011 TNF-a ELISA R&D Systems Cat#DY 410 IL-6 ELISA R&D Systems Cat#DY 406 IL-1b ELISA R&D Systems Cat#DY 401 IFN-b ELISA R&D Systems Cat#DY 8234-05 MPO ELISA Abcam Cat#ab155458 Bio-Rad Protein Assay (Bradford assay) Bio-Rad Cat#5000001 TRIzolTM Reagent Thermo Fisher Scientific Cat#15596026 NativePAGETM Sample Prep Kit Thermo Fisher Scientific Cat#BN2008 NativeMarkTM Unstained Protein Standard Thermo Fisher Scientific Cat#LC0725 NativePAGETM 4 to 16%, Bis-Tris, 1.0 mm, Mini Protein Gels Thermo Fisher Scientific Cat#BN1002BOX NativePAGETM Running Buffer Kit Thermo Fisher Scientific Cat#BN2007 NuPAGETM Transfer Buffer Thermo Fisher Scientific Cat#NP0006 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 IGEPAL CA-630 Sigma Cat#I8896 4–20% Mini-PROTEAN TGXTM Precast Protein Gels Bio-Rad Cat#4561096 Deposited data Targeted metabolomics analysis of WT and IFNAR1 deficient murine bone marrow-derived macrophages following methicillin-resistant Staphylococcus aureus infection [Dataset] This paper https://doi.org/10.7302/h15v-j558 RNAseq analysis of Tlr2/4/9 / iBMDM and paired WT controls [Dataset] This paper GEO accession # GSE271727 Experimental models: Cell lines Tlr2/4/9 / iBMDM and paired WT controls Derived in our lab from mice obtained from the laboratory of Dr. Tod Merkel, FDA20 Ifnar1 / iBMDM and paired WT controls Derived in our lab from mice obtained from the Jackson Laboratory strain #010830 (Continued on next page) Cell Reports 43, 114607, August 27, 2024 19
Polymerase Chain Reaction:Article Title: Type I interferon governs immunometabolic checkpoints that coordinate inflammation during Staphylococcal infection.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lysostaphin Sigma Cat#L7386 Critical commercial assays Cut & Run kit Cell Signaling Cat#86652 Lactate assay Cayman Chemical Cat#600450 Greiss reagent Abcam Cat#ab234044 Mito Stress Test Agilent Cat#103015-100 LookOut Mycoplasma PCR Detection Kit Sigma Cat#MP0035 MitoTracker Green Thermo Fisher Scientific Cat#M7514 MitoTracker Deep Red Thermo Fisher Scientific Cat#M22426 Propidium iodide Bio-Rad Cat#1351101 Hoechst Thermo Fisher Scientific Cat#H3570 DAPI Thermo Fisher Scientific Cat#EN62248 PowerUP SYBR Green PCR master mix Thermo Fisher Scientific Cat#A25742 RNAeasy Plus Mini kit Qiagen Cat#74134 iScript cDNA synthesis kit Bio-Rad Cat#1708890 BioMasher II homogenizers Thermo Fisher Scientific Cat#50-136-7690 Halt protease and phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RevertTM 700 Total Protein Stain for Western Blot Normalization LI-COR Cat#926-11011 TNF-a ELISA R&D Systems Cat#DY 410 IL-6 ELISA R&D Systems Cat#DY 406 IL-1b ELISA R&D Systems Cat#DY 401 IFN-b ELISA R&D Systems Cat#DY 8234-05 MPO ELISA Abcam Cat#ab155458 Bio-Rad Protein Assay (Bradford assay) Bio-Rad Cat#5000001 TRIzolTM Reagent Thermo Fisher Scientific Cat#15596026 NativePAGETM Sample Prep Kit Thermo Fisher Scientific Cat#BN2008 NativeMarkTM Unstained Protein Standard Thermo Fisher Scientific Cat#LC0725 NativePAGETM 4 to 16%, Bis-Tris, 1.0 mm, Mini Protein Gels Thermo Fisher Scientific Cat#BN1002BOX NativePAGETM Running Buffer Kit Thermo Fisher Scientific Cat#BN2007 NuPAGETM Transfer Buffer Thermo Fisher Scientific Cat#NP0006 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 IGEPAL CA-630 Sigma Cat#I8896 4–20% Mini-PROTEAN TGXTM Precast Protein Gels Bio-Rad Cat#4561096 Deposited data Targeted metabolomics analysis of WT and IFNAR1 deficient murine bone marrow-derived macrophages following methicillin-resistant Staphylococcus aureus infection [Dataset] This paper https://doi.org/10.7302/h15v-j558 RNAseq analysis of Tlr2/4/9 / iBMDM and paired WT controls [Dataset] This paper GEO accession # GSE271727 Experimental models: Cell lines Tlr2/4/9 / iBMDM and paired WT controls Derived in our lab from mice obtained from the laboratory of Dr. Tod Merkel, FDA20 Ifnar1 / iBMDM and paired WT controls Derived in our lab from mice obtained from the Jackson Laboratory strain #010830 (Continued on next page) Cell Reports 43, 114607, August 27, 2024 19
SYBR Green Assay:Article Title: Type I interferon governs immunometabolic checkpoints that coordinate inflammation during Staphylococcal infection.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lysostaphin Sigma Cat#L7386 Critical commercial assays Cut & Run kit Cell Signaling Cat#86652 Lactate assay Cayman Chemical Cat#600450 Greiss reagent Abcam Cat#ab234044 Mito Stress Test Agilent Cat#103015-100 LookOut Mycoplasma PCR Detection Kit Sigma Cat#MP0035 MitoTracker Green Thermo Fisher Scientific Cat#M7514 MitoTracker Deep Red Thermo Fisher Scientific Cat#M22426 Propidium iodide Bio-Rad Cat#1351101 Hoechst Thermo Fisher Scientific Cat#H3570 DAPI Thermo Fisher Scientific Cat#EN62248 PowerUP SYBR Green PCR master mix Thermo Fisher Scientific Cat#A25742 RNAeasy Plus Mini kit Qiagen Cat#74134 iScript cDNA synthesis kit Bio-Rad Cat#1708890 BioMasher II homogenizers Thermo Fisher Scientific Cat#50-136-7690 Halt protease and phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RevertTM 700 Total Protein Stain for Western Blot Normalization LI-COR Cat#926-11011 TNF-a ELISA R&D Systems Cat#DY 410 IL-6 ELISA R&D Systems Cat#DY 406 IL-1b ELISA R&D Systems Cat#DY 401 IFN-b ELISA R&D Systems Cat#DY 8234-05 MPO ELISA Abcam Cat#ab155458 Bio-Rad Protein Assay (Bradford assay) Bio-Rad Cat#5000001 TRIzolTM Reagent Thermo Fisher Scientific Cat#15596026 NativePAGETM Sample Prep Kit Thermo Fisher Scientific Cat#BN2008 NativeMarkTM Unstained Protein Standard Thermo Fisher Scientific Cat#LC0725 NativePAGETM 4 to 16%, Bis-Tris, 1.0 mm, Mini Protein Gels Thermo Fisher Scientific Cat#BN1002BOX NativePAGETM Running Buffer Kit Thermo Fisher Scientific Cat#BN2007 NuPAGETM Transfer Buffer Thermo Fisher Scientific Cat#NP0006 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 IGEPAL CA-630 Sigma Cat#I8896 4–20% Mini-PROTEAN TGXTM Precast Protein Gels Bio-Rad Cat#4561096 Deposited data Targeted metabolomics analysis of WT and IFNAR1 deficient murine bone marrow-derived macrophages following methicillin-resistant Staphylococcus aureus infection [Dataset] This paper https://doi.org/10.7302/h15v-j558 RNAseq analysis of Tlr2/4/9 / iBMDM and paired WT controls [Dataset] This paper GEO accession # GSE271727 Experimental models: Cell lines Tlr2/4/9 / iBMDM and paired WT controls Derived in our lab from mice obtained from the laboratory of Dr. Tod Merkel, FDA20 Ifnar1 / iBMDM and paired WT controls Derived in our lab from mice obtained from the Jackson Laboratory strain #010830 (Continued on next page) Cell Reports 43, 114607, August 27, 2024 19
cDNA Synthesis:Article Title: Type I interferon governs immunometabolic checkpoints that coordinate inflammation during Staphylococcal infection.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lysostaphin Sigma Cat#L7386 Critical commercial assays Cut & Run kit Cell Signaling Cat#86652 Lactate assay Cayman Chemical Cat#600450 Greiss reagent Abcam Cat#ab234044 Mito Stress Test Agilent Cat#103015-100 LookOut Mycoplasma PCR Detection Kit Sigma Cat#MP0035 MitoTracker Green Thermo Fisher Scientific Cat#M7514 MitoTracker Deep Red Thermo Fisher Scientific Cat#M22426 Propidium iodide Bio-Rad Cat#1351101 Hoechst Thermo Fisher Scientific Cat#H3570 DAPI Thermo Fisher Scientific Cat#EN62248 PowerUP SYBR Green PCR master mix Thermo Fisher Scientific Cat#A25742 RNAeasy Plus Mini kit Qiagen Cat#74134 iScript cDNA synthesis kit Bio-Rad Cat#1708890 BioMasher II homogenizers Thermo Fisher Scientific Cat#50-136-7690 Halt protease and phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RevertTM 700 Total Protein Stain for Western Blot Normalization LI-COR Cat#926-11011 TNF-a ELISA R&D Systems Cat#DY 410 IL-6 ELISA R&D Systems Cat#DY 406 IL-1b ELISA R&D Systems Cat#DY 401 IFN-b ELISA R&D Systems Cat#DY 8234-05 MPO ELISA Abcam Cat#ab155458 Bio-Rad Protein Assay (Bradford assay) Bio-Rad Cat#5000001 TRIzolTM Reagent Thermo Fisher Scientific Cat#15596026 NativePAGETM Sample Prep Kit Thermo Fisher Scientific Cat#BN2008 NativeMarkTM Unstained Protein Standard Thermo Fisher Scientific Cat#LC0725 NativePAGETM 4 to 16%, Bis-Tris, 1.0 mm, Mini Protein Gels Thermo Fisher Scientific Cat#BN1002BOX NativePAGETM Running Buffer Kit Thermo Fisher Scientific Cat#BN2007 NuPAGETM Transfer Buffer Thermo Fisher Scientific Cat#NP0006 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 IGEPAL CA-630 Sigma Cat#I8896 4–20% Mini-PROTEAN TGXTM Precast Protein Gels Bio-Rad Cat#4561096 Deposited data Targeted metabolomics analysis of WT and IFNAR1 deficient murine bone marrow-derived macrophages following methicillin-resistant Staphylococcus aureus infection [Dataset] This paper https://doi.org/10.7302/h15v-j558 RNAseq analysis of Tlr2/4/9 / iBMDM and paired WT controls [Dataset] This paper GEO accession # GSE271727 Experimental models: Cell lines Tlr2/4/9 / iBMDM and paired WT controls Derived in our lab from mice obtained from the laboratory of Dr. Tod Merkel, FDA20 Ifnar1 / iBMDM and paired WT controls Derived in our lab from mice obtained from the Jackson Laboratory strain #010830 (Continued on next page) Cell Reports 43, 114607, August 27, 2024 19
Staining:Article Title: Type I interferon governs immunometabolic checkpoints that coordinate inflammation during Staphylococcal infection.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lysostaphin Sigma Cat#L7386 Critical commercial assays Cut & Run kit Cell Signaling Cat#86652 Lactate assay Cayman Chemical Cat#600450 Greiss reagent Abcam Cat#ab234044 Mito Stress Test Agilent Cat#103015-100 LookOut Mycoplasma PCR Detection Kit Sigma Cat#MP0035 MitoTracker Green Thermo Fisher Scientific Cat#M7514 MitoTracker Deep Red Thermo Fisher Scientific Cat#M22426 Propidium iodide Bio-Rad Cat#1351101 Hoechst Thermo Fisher Scientific Cat#H3570 DAPI Thermo Fisher Scientific Cat#EN62248 PowerUP SYBR Green PCR master mix Thermo Fisher Scientific Cat#A25742 RNAeasy Plus Mini kit Qiagen Cat#74134 iScript cDNA synthesis kit Bio-Rad Cat#1708890 BioMasher II homogenizers Thermo Fisher Scientific Cat#50-136-7690 Halt protease and phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RevertTM 700 Total Protein Stain for Western Blot Normalization LI-COR Cat#926-11011 TNF-a ELISA R&D Systems Cat#DY 410 IL-6 ELISA R&D Systems Cat#DY 406 IL-1b ELISA R&D Systems Cat#DY 401 IFN-b ELISA R&D Systems Cat#DY 8234-05 MPO ELISA Abcam Cat#ab155458 Bio-Rad Protein Assay (Bradford assay) Bio-Rad Cat#5000001 TRIzolTM Reagent Thermo Fisher Scientific Cat#15596026 NativePAGETM Sample Prep Kit Thermo Fisher Scientific Cat#BN2008 NativeMarkTM Unstained Protein Standard Thermo Fisher Scientific Cat#LC0725 NativePAGETM 4 to 16%, Bis-Tris, 1.0 mm, Mini Protein Gels Thermo Fisher Scientific Cat#BN1002BOX NativePAGETM Running Buffer Kit Thermo Fisher Scientific Cat#BN2007 NuPAGETM Transfer Buffer Thermo Fisher Scientific Cat#NP0006 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 IGEPAL CA-630 Sigma Cat#I8896 4–20% Mini-PROTEAN TGXTM Precast Protein Gels Bio-Rad Cat#4561096 Deposited data Targeted metabolomics analysis of WT and IFNAR1 deficient murine bone marrow-derived macrophages following methicillin-resistant Staphylococcus aureus infection [Dataset] This paper https://doi.org/10.7302/h15v-j558 RNAseq analysis of Tlr2/4/9 / iBMDM and paired WT controls [Dataset] This paper GEO accession # GSE271727 Experimental models: Cell lines Tlr2/4/9 / iBMDM and paired WT controls Derived in our lab from mice obtained from the laboratory of Dr. Tod Merkel, FDA20 Ifnar1 / iBMDM and paired WT controls Derived in our lab from mice obtained from the Jackson Laboratory strain #010830 (Continued on next page) Cell Reports 43, 114607, August 27, 2024 19
Western Blot:Article Title: Type I interferon governs immunometabolic checkpoints that coordinate inflammation during Staphylococcal infection.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lysostaphin Sigma Cat#L7386 Critical commercial assays Cut & Run kit Cell Signaling Cat#86652 Lactate assay Cayman Chemical Cat#600450 Greiss reagent Abcam Cat#ab234044 Mito Stress Test Agilent Cat#103015-100 LookOut Mycoplasma PCR Detection Kit Sigma Cat#MP0035 MitoTracker Green Thermo Fisher Scientific Cat#M7514 MitoTracker Deep Red Thermo Fisher Scientific Cat#M22426 Propidium iodide Bio-Rad Cat#1351101 Hoechst Thermo Fisher Scientific Cat#H3570 DAPI Thermo Fisher Scientific Cat#EN62248 PowerUP SYBR Green PCR master mix Thermo Fisher Scientific Cat#A25742 RNAeasy Plus Mini kit Qiagen Cat#74134 iScript cDNA synthesis kit Bio-Rad Cat#1708890 BioMasher II homogenizers Thermo Fisher Scientific Cat#50-136-7690 Halt protease and phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RevertTM 700 Total Protein Stain for Western Blot Normalization LI-COR Cat#926-11011 TNF-a ELISA R&D Systems Cat#DY 410 IL-6 ELISA R&D Systems Cat#DY 406 IL-1b ELISA R&D Systems Cat#DY 401 IFN-b ELISA R&D Systems Cat#DY 8234-05 MPO ELISA Abcam Cat#ab155458 Bio-Rad Protein Assay (Bradford assay) Bio-Rad Cat#5000001 TRIzolTM Reagent Thermo Fisher Scientific Cat#15596026 NativePAGETM Sample Prep Kit Thermo Fisher Scientific Cat#BN2008 NativeMarkTM Unstained Protein Standard Thermo Fisher Scientific Cat#LC0725 NativePAGETM 4 to 16%, Bis-Tris, 1.0 mm, Mini Protein Gels Thermo Fisher Scientific Cat#BN1002BOX NativePAGETM Running Buffer Kit Thermo Fisher Scientific Cat#BN2007 NuPAGETM Transfer Buffer Thermo Fisher Scientific Cat#NP0006 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 IGEPAL CA-630 Sigma Cat#I8896 4–20% Mini-PROTEAN TGXTM Precast Protein Gels Bio-Rad Cat#4561096 Deposited data Targeted metabolomics analysis of WT and IFNAR1 deficient murine bone marrow-derived macrophages following methicillin-resistant Staphylococcus aureus infection [Dataset] This paper https://doi.org/10.7302/h15v-j558 RNAseq analysis of Tlr2/4/9 / iBMDM and paired WT controls [Dataset] This paper GEO accession # GSE271727 Experimental models: Cell lines Tlr2/4/9 / iBMDM and paired WT controls Derived in our lab from mice obtained from the laboratory of Dr. Tod Merkel, FDA20 Ifnar1 / iBMDM and paired WT controls Derived in our lab from mice obtained from the Jackson Laboratory strain #010830 (Continued on next page) Cell Reports 43, 114607, August 27, 2024 19
Enzyme-linked Immunosorbent Assay:Article Title: Type I interferon governs immunometabolic checkpoints that coordinate inflammation during Staphylococcal infection.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lysostaphin Sigma Cat#L7386 Critical commercial assays Cut & Run kit Cell Signaling Cat#86652 Lactate assay Cayman Chemical Cat#600450 Greiss reagent Abcam Cat#ab234044 Mito Stress Test Agilent Cat#103015-100 LookOut Mycoplasma PCR Detection Kit Sigma Cat#MP0035 MitoTracker Green Thermo Fisher Scientific Cat#M7514 MitoTracker Deep Red Thermo Fisher Scientific Cat#M22426 Propidium iodide Bio-Rad Cat#1351101 Hoechst Thermo Fisher Scientific Cat#H3570 DAPI Thermo Fisher Scientific Cat#EN62248 PowerUP SYBR Green PCR master mix Thermo Fisher Scientific Cat#A25742 RNAeasy Plus Mini kit Qiagen Cat#74134 iScript cDNA synthesis kit Bio-Rad Cat#1708890 BioMasher II homogenizers Thermo Fisher Scientific Cat#50-136-7690 Halt protease and phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RevertTM 700 Total Protein Stain for Western Blot Normalization LI-COR Cat#926-11011 TNF-a ELISA R&D Systems Cat#DY 410 IL-6 ELISA R&D Systems Cat#DY 406 IL-1b ELISA R&D Systems Cat#DY 401 IFN-b ELISA R&D Systems Cat#DY 8234-05 MPO ELISA Abcam Cat#ab155458 Bio-Rad Protein Assay (Bradford assay) Bio-Rad Cat#5000001 TRIzolTM Reagent Thermo Fisher Scientific Cat#15596026 NativePAGETM Sample Prep Kit Thermo Fisher Scientific Cat#BN2008 NativeMarkTM Unstained Protein Standard Thermo Fisher Scientific Cat#LC0725 NativePAGETM 4 to 16%, Bis-Tris, 1.0 mm, Mini Protein Gels Thermo Fisher Scientific Cat#BN1002BOX NativePAGETM Running Buffer Kit Thermo Fisher Scientific Cat#BN2007 NuPAGETM Transfer Buffer Thermo Fisher Scientific Cat#NP0006 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 IGEPAL CA-630 Sigma Cat#I8896 4–20% Mini-PROTEAN TGXTM Precast Protein Gels Bio-Rad Cat#4561096 Deposited data Targeted metabolomics analysis of WT and IFNAR1 deficient murine bone marrow-derived macrophages following methicillin-resistant Staphylococcus aureus infection [Dataset] This paper https://doi.org/10.7302/h15v-j558 RNAseq analysis of Tlr2/4/9 / iBMDM and paired WT controls [Dataset] This paper GEO accession # GSE271727 Experimental models: Cell lines Tlr2/4/9 / iBMDM and paired WT controls Derived in our lab from mice obtained from the laboratory of Dr. Tod Merkel, FDA20 Ifnar1 / iBMDM and paired WT controls Derived in our lab from mice obtained from the Jackson Laboratory strain #010830 (Continued on next page) Cell Reports 43, 114607, August 27, 2024 19
Bradford Assay:Article Title: Type I interferon governs immunometabolic checkpoints that coordinate inflammation during Staphylococcal infection.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lysostaphin Sigma Cat#L7386 Critical commercial assays Cut & Run kit Cell Signaling Cat#86652 Lactate assay Cayman Chemical Cat#600450 Greiss reagent Abcam Cat#ab234044 Mito Stress Test Agilent Cat#103015-100 LookOut Mycoplasma PCR Detection Kit Sigma Cat#MP0035 MitoTracker Green Thermo Fisher Scientific Cat#M7514 MitoTracker Deep Red Thermo Fisher Scientific Cat#M22426 Propidium iodide Bio-Rad Cat#1351101 Hoechst Thermo Fisher Scientific Cat#H3570 DAPI Thermo Fisher Scientific Cat#EN62248 PowerUP SYBR Green PCR master mix Thermo Fisher Scientific Cat#A25742 RNAeasy Plus Mini kit Qiagen Cat#74134 iScript cDNA synthesis kit Bio-Rad Cat#1708890 BioMasher II homogenizers Thermo Fisher Scientific Cat#50-136-7690 Halt protease and phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RevertTM 700 Total Protein Stain for Western Blot Normalization LI-COR Cat#926-11011 TNF-a ELISA R&D Systems Cat#DY 410 IL-6 ELISA R&D Systems Cat#DY 406 IL-1b ELISA R&D Systems Cat#DY 401 IFN-b ELISA R&D Systems Cat#DY 8234-05 MPO ELISA Abcam Cat#ab155458 Bio-Rad Protein Assay (Bradford assay) Bio-Rad Cat#5000001 TRIzolTM Reagent Thermo Fisher Scientific Cat#15596026 NativePAGETM Sample Prep Kit Thermo Fisher Scientific Cat#BN2008 NativeMarkTM Unstained Protein Standard Thermo Fisher Scientific Cat#LC0725 NativePAGETM 4 to 16%, Bis-Tris, 1.0 mm, Mini Protein Gels Thermo Fisher Scientific Cat#BN1002BOX NativePAGETM Running Buffer Kit Thermo Fisher Scientific Cat#BN2007 NuPAGETM Transfer Buffer Thermo Fisher Scientific Cat#NP0006 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 IGEPAL CA-630 Sigma Cat#I8896 4–20% Mini-PROTEAN TGXTM Precast Protein Gels Bio-Rad Cat#4561096 Deposited data Targeted metabolomics analysis of WT and IFNAR1 deficient murine bone marrow-derived macrophages following methicillin-resistant Staphylococcus aureus infection [Dataset] This paper https://doi.org/10.7302/h15v-j558 RNAseq analysis of Tlr2/4/9 / iBMDM and paired WT controls [Dataset] This paper GEO accession # GSE271727 Experimental models: Cell lines Tlr2/4/9 / iBMDM and paired WT controls Derived in our lab from mice obtained from the laboratory of Dr. Tod Merkel, FDA20 Ifnar1 / iBMDM and paired WT controls Derived in our lab from mice obtained from the Jackson Laboratory strain #010830 (Continued on next page) Cell Reports 43, 114607, August 27, 2024 19
Sample Prep:Article Title: Type I interferon governs immunometabolic checkpoints that coordinate inflammation during Staphylococcal infection.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lysostaphin Sigma Cat#L7386 Critical commercial assays Cut & Run kit Cell Signaling Cat#86652 Lactate assay Cayman Chemical Cat#600450 Greiss reagent Abcam Cat#ab234044 Mito Stress Test Agilent Cat#103015-100 LookOut Mycoplasma PCR Detection Kit Sigma Cat#MP0035 MitoTracker Green Thermo Fisher Scientific Cat#M7514 MitoTracker Deep Red Thermo Fisher Scientific Cat#M22426 Propidium iodide Bio-Rad Cat#1351101 Hoechst Thermo Fisher Scientific Cat#H3570 DAPI Thermo Fisher Scientific Cat#EN62248 PowerUP SYBR Green PCR master mix Thermo Fisher Scientific Cat#A25742 RNAeasy Plus Mini kit Qiagen Cat#74134 iScript cDNA synthesis kit Bio-Rad Cat#1708890 BioMasher II homogenizers Thermo Fisher Scientific Cat#50-136-7690 Halt protease and phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RevertTM 700 Total Protein Stain for Western Blot Normalization LI-COR Cat#926-11011 TNF-a ELISA R&D Systems Cat#DY 410 IL-6 ELISA R&D Systems Cat#DY 406 IL-1b ELISA R&D Systems Cat#DY 401 IFN-b ELISA R&D Systems Cat#DY 8234-05 MPO ELISA Abcam Cat#ab155458 Bio-Rad Protein Assay (Bradford assay) Bio-Rad Cat#5000001 TRIzolTM Reagent Thermo Fisher Scientific Cat#15596026 NativePAGETM Sample Prep Kit Thermo Fisher Scientific Cat#BN2008 NativeMarkTM Unstained Protein Standard Thermo Fisher Scientific Cat#LC0725 NativePAGETM 4 to 16%, Bis-Tris, 1.0 mm, Mini Protein Gels Thermo Fisher Scientific Cat#BN1002BOX NativePAGETM Running Buffer Kit Thermo Fisher Scientific Cat#BN2007 NuPAGETM Transfer Buffer Thermo Fisher Scientific Cat#NP0006 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 IGEPAL CA-630 Sigma Cat#I8896 4–20% Mini-PROTEAN TGXTM Precast Protein Gels Bio-Rad Cat#4561096 Deposited data Targeted metabolomics analysis of WT and IFNAR1 deficient murine bone marrow-derived macrophages following methicillin-resistant Staphylococcus aureus infection [Dataset] This paper https://doi.org/10.7302/h15v-j558 RNAseq analysis of Tlr2/4/9 / iBMDM and paired WT controls [Dataset] This paper GEO accession # GSE271727 Experimental models: Cell lines Tlr2/4/9 / iBMDM and paired WT controls Derived in our lab from mice obtained from the laboratory of Dr. Tod Merkel, FDA20 Ifnar1 / iBMDM and paired WT controls Derived in our lab from mice obtained from the Jackson Laboratory strain #010830 (Continued on next page) Cell Reports 43, 114607, August 27, 2024 19
Infection:Article Title: Type I interferon governs immunometabolic checkpoints that coordinate inflammation during Staphylococcal infection.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lysostaphin Sigma Cat#L7386 Critical commercial assays Cut & Run kit Cell Signaling Cat#86652 Lactate assay Cayman Chemical Cat#600450 Greiss reagent Abcam Cat#ab234044 Mito Stress Test Agilent Cat#103015-100 LookOut Mycoplasma PCR Detection Kit Sigma Cat#MP0035 MitoTracker Green Thermo Fisher Scientific Cat#M7514 MitoTracker Deep Red Thermo Fisher Scientific Cat#M22426 Propidium iodide Bio-Rad Cat#1351101 Hoechst Thermo Fisher Scientific Cat#H3570 DAPI Thermo Fisher Scientific Cat#EN62248 PowerUP SYBR Green PCR master mix Thermo Fisher Scientific Cat#A25742 RNAeasy Plus Mini kit Qiagen Cat#74134 iScript cDNA synthesis kit Bio-Rad Cat#1708890 BioMasher II homogenizers Thermo Fisher Scientific Cat#50-136-7690 Halt protease and phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RevertTM 700 Total Protein Stain for Western Blot Normalization LI-COR Cat#926-11011 TNF-a ELISA R&D Systems Cat#DY 410 IL-6 ELISA R&D Systems Cat#DY 406 IL-1b ELISA R&D Systems Cat#DY 401 IFN-b ELISA R&D Systems Cat#DY 8234-05 MPO ELISA Abcam Cat#ab155458 Bio-Rad Protein Assay (Bradford assay) Bio-Rad Cat#5000001 TRIzolTM Reagent Thermo Fisher Scientific Cat#15596026 NativePAGETM Sample Prep Kit Thermo Fisher Scientific Cat#BN2008 NativeMarkTM Unstained Protein Standard Thermo Fisher Scientific Cat#LC0725 NativePAGETM 4 to 16%, Bis-Tris, 1.0 mm, Mini Protein Gels Thermo Fisher Scientific Cat#BN1002BOX NativePAGETM Running Buffer Kit Thermo Fisher Scientific Cat#BN2007 NuPAGETM Transfer Buffer Thermo Fisher Scientific Cat#NP0006 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 IGEPAL CA-630 Sigma Cat#I8896 4–20% Mini-PROTEAN TGXTM Precast Protein Gels Bio-Rad Cat#4561096 Deposited data Targeted metabolomics analysis of WT and IFNAR1 deficient murine bone marrow-derived macrophages following methicillin-resistant Staphylococcus aureus infection [Dataset] This paper https://doi.org/10.7302/h15v-j558 RNAseq analysis of Tlr2/4/9 / iBMDM and paired WT controls [Dataset] This paper GEO accession # GSE271727 Experimental models: Cell lines Tlr2/4/9 / iBMDM and paired WT controls Derived in our lab from mice obtained from the laboratory of Dr. Tod Merkel, FDA20 Ifnar1 / iBMDM and paired WT controls Derived in our lab from mice obtained from the Jackson Laboratory strain #010830 (Continued on next page) Cell Reports 43, 114607, August 27, 2024 19
Derivative Assay:Article Title: Type I interferon governs immunometabolic checkpoints that coordinate inflammation during Staphylococcal infection.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lysostaphin Sigma Cat#L7386 Critical commercial assays Cut & Run kit Cell Signaling Cat#86652 Lactate assay Cayman Chemical Cat#600450 Greiss reagent Abcam Cat#ab234044 Mito Stress Test Agilent Cat#103015-100 LookOut Mycoplasma PCR Detection Kit Sigma Cat#MP0035 MitoTracker Green Thermo Fisher Scientific Cat#M7514 MitoTracker Deep Red Thermo Fisher Scientific Cat#M22426 Propidium iodide Bio-Rad Cat#1351101 Hoechst Thermo Fisher Scientific Cat#H3570 DAPI Thermo Fisher Scientific Cat#EN62248 PowerUP SYBR Green PCR master mix Thermo Fisher Scientific Cat#A25742 RNAeasy Plus Mini kit Qiagen Cat#74134 iScript cDNA synthesis kit Bio-Rad Cat#1708890 BioMasher II homogenizers Thermo Fisher Scientific Cat#50-136-7690 Halt protease and phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RevertTM 700 Total Protein Stain for Western Blot Normalization LI-COR Cat#926-11011 TNF-a ELISA R&D Systems Cat#DY 410 IL-6 ELISA R&D Systems Cat#DY 406 IL-1b ELISA R&D Systems Cat#DY 401 IFN-b ELISA R&D Systems Cat#DY 8234-05 MPO ELISA Abcam Cat#ab155458 Bio-Rad Protein Assay (Bradford assay) Bio-Rad Cat#5000001 TRIzolTM Reagent Thermo Fisher Scientific Cat#15596026 NativePAGETM Sample Prep Kit Thermo Fisher Scientific Cat#BN2008 NativeMarkTM Unstained Protein Standard Thermo Fisher Scientific Cat#LC0725 NativePAGETM 4 to 16%, Bis-Tris, 1.0 mm, Mini Protein Gels Thermo Fisher Scientific Cat#BN1002BOX NativePAGETM Running Buffer Kit Thermo Fisher Scientific Cat#BN2007 NuPAGETM Transfer Buffer Thermo Fisher Scientific Cat#NP0006 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 IGEPAL CA-630 Sigma Cat#I8896 4–20% Mini-PROTEAN TGXTM Precast Protein Gels Bio-Rad Cat#4561096 Deposited data Targeted metabolomics analysis of WT and IFNAR1 deficient murine bone marrow-derived macrophages following methicillin-resistant Staphylococcus aureus infection [Dataset] This paper https://doi.org/10.7302/h15v-j558 RNAseq analysis of Tlr2/4/9 / iBMDM and paired WT controls [Dataset] This paper GEO accession # GSE271727 Experimental models: Cell lines Tlr2/4/9 / iBMDM and paired WT controls Derived in our lab from mice obtained from the laboratory of Dr. Tod Merkel, FDA20 Ifnar1 / iBMDM and paired WT controls Derived in our lab from mice obtained from the Jackson Laboratory strain #010830 (Continued on next page) Cell Reports 43, 114607, August 27, 2024 19
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